APPROACHES: We have developed an interdisciplinary strategy combining electrophysiological, pharmacological and anatomical techniques applied to brain tissue of wild-type and genetically modified mice, and also to lesion-generated animal models of brain diseases.
TECHNIQUES: The methods used and handled efficiently in the laboratory are: High resolution immunoelectron microscopy; Electrophysiology ex vivo in brain slices (extracellular field recordings and patch-clamp); Immunocytochemistry for conventional light and confocal microscopy; Tract-tracing; Behavioural tests; Organotypic cultures.
