Pinus canariensis plant regeneration through somatic embryogenesis

  1. Ander Castander-Olarieta 1
  2. Paloma Moncaleán 1
  3. Itziar A. Montalbán 1
  1. 1 Department of Forestry Science, NEIKER-Tecnalia, Arkaute
Revista:
Forest systems

ISSN: 2171-5068

Año de publicación: 2020

Volumen: 29

Número: 1

Páginas: 61-66

Tipo: Artículo

DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Forest systems

Resumen

Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis. Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain). Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions. Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth. Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.

€ Ver financiación

Información de financiación

MINECO (Spanish Government) AGL2016-76143-C4-3R CYTED P117RT0522 DECO (Basque government) Ayudas de formaci?n a j?venes investigadores y tecn?logos Special mention to Enrique Surroca, who actively helped with the collection of plant material.

Financiadores

Referencias bibliográficas

  • Aitken-Christie J, Singh AP, Davies H, 1988. Multiplication of meristematic tissue: a new tissue culture system for radiata pine. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum, New York, pp 413-432. https://doi.org/10.1007/978-1-4613-1661-9_26
  • Bornman CH, 1983. Possibilities and constraits in the regeneration of trees from cotyledonary needles of Picea abies in vitro. Physiol Plant 57: 5-16. https://doi.org/10.1111/j.1399-3054.1983.tb00722.x
  • Breton D, Harvengt L, Trontin J-F, Bouvet A, Favre J-M, 2005. High subculture frequency, maltose-based and hormone-free medium sustained early development of somatic embryos in maritime pine. Vitr Cell Dev Biol - Plant 41: 494-504. https://doi.org/10.1079/IVP2005671
  • Brito P, Grams TEE., Matysssek R, Jimenez MS, Gonzalez-Rodríguez AM, Oberhuber W, Wieser G, 2016. Increased water use efficiency does not prevent growth decline of Pinus canariensis in a semi-arid treeline ecotone in Tenerife, Canary Islands (Spain). Ann For Sci 73: 741-749. https://doi.org/10.1007/s13595-016-0562-5
  • Carneros E, Toribio M, Celestino C, 2017. Effect of ABA, the auxin antagonist PCIB and partial desiccation on stone pine somatic embryo maturation. Plant Cell Tiss Org Cult 131: 445-458. https://doi.org/10.1007/s11240-017-1296-2
  • Castander-Olarieta A, Montalbán IA, De Medeiros Oliveira E, Dell'Aversana E, D'Amelia L, Carillo P, Steiner N, Fraga HPF, Guerra MP, Goicoa T, et al., 2019. Effect of thermal stress on tissue ultrastructure and metabolite profiles during initiation of radiata pine somatic embryogenesis. Front Plant Sci 9: 1-16. https://doi.org/10.3389/fpls.2018.02004
  • Durán J, Rodríguez A, Fernández-Palacios JM, Gallardo A, 2010. Long-term decrease of organic and inorganic nitrogen concentrations due to pine forest wildfire. Ann For Sci 67. https://doi.org/10.1051/forest/2009100
  • García-Mendiguren O, Montalbán IA, Goicoa T, Ugarte MD, Moncaleán P, 2016. Environmental conditions at the initial stages of Pinus radiata somatic embryogenesis affect the production of somatic embryos. Trees - Struct Funct 30: 949-958. https://doi.org/10.1007/s00468-015-1336-7
  • García-Mendiguren O, Montalbán IA, Goicoa T, Ugarte MD, Moncaleán P, 2017. Are we able to modulate the response of somatic embryos of pines to drought stress?. Acta Hortic 1155: 77-84. https://doi.org/10.17660/ActaHortic.2017.1155.10
  • Garin E, Bernier-Cardou M, Isabel N, Klimaszewska K, Plourde A, 2000. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations. Plant Cell Tiss Org Cult 62: 27-37. https://doi.org/10.1023/A:1006402215457
  • Gupta PK, Durzan DJ, 1985. Shoot multiplication from mature trees of Douglas fir and sugar pine. Plant Cell Rep 4: 177-179. https://doi.org/10.1007/BF00269282
  • López De Heredia U, López R, Collada C, Emerson BC, Gil L, 2014. Signatures of volcanism and aridity in the evolution of an insular pine (Pinus canariensis Chr. Sm. Ex DC in Buch). Heredity (Edinb) 113: 240-249. https://doi.org/10.1038/hdy.2014.22
  • Martinez Pulido CM, Harry IS, Thorpe TA, 1990. In vitro regeneration of plantlets of Canary Island pine (Pinus canariensis). Can J For Res 20: 1200-1211. https://doi.org/10.1139/x90-159
  • Martinez Pulido CM, Harry IS, Thorpe TA, 1992. Optimization of bud induction in cotyledonary explants of Pinus canariensis. Plant Cell Tiss Org Cult 29: 247-255. https://doi.org/10.1007/BF00034360
  • Martinez Pulido CM, Harry IS, Thorpe TA, 1994. Effect of various bud induction treatments on elongation and rooting of adventitious shoots of Canary Island pine (Pinus canariensis). Plant Cell Tiss Org Cult 39: 225-230. https://doi.org/10.1007/BF00035974
  • Montalbán IA, De Diego N, Moncaleán P, 2010. Bottlenecks in Pinus radiata somatic embryogenesis: Improving maturation and germination. Trees - Struct Funct 24: 1061-1071. https://doi.org/10.1007/s00468-010-0477-y
  • Montalbán IA, De Diego N, Moncaleán P, 2012. Enhancing initiation and proliferation in radiata pine (Pinus radiata D. Don) somatic embryogenesis through seed family screening, zygotic embryo staging and media adjustments. Acta Physiol Plant 34: 451-460. https://doi.org/10.1007/s11738-011-0841-6
  • Montalbán IA, Setién-Olarra A, Hargreaves CL, Moncaleán P, 2013. Somatic embryogenesis in Pinus halepensis Mill.: An important ecological species from the Mediterranean forest. Trees - Struct Funct 27: 1339-1351. https://doi.org/10.1007/s00468-013-0882-0
  • Montalbán IA, García-Mendiguren O, Goicoa T, Ugarte MD, Moncaleán P, 2014. Cold storage of initial plant material affects positively somatic embryogenesis in Pinus radiata. New For 46: 309-317. https://doi.org/10.1007/s11056-014-9457-1
  • Montalbán IA, García-Mendiguren O, Moncaleán P, 2016. Somatic embryogenesis in Pinus spp. In: Germana M, Lambardi M (eds) In vitro embryogenesis in higher plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, pp 405-415. https://doi.org/10.1007/978-1-4939-3061-6_21
  • Montalbán IA, Moncaleán P, 2018. Rooting of Pinus radiata somatic embryos: factors involved in the success of the process. J For Res 30: 65-71. https://doi.org/10.1007/s11676-018-0618-5
  • Otto R, García-del-Rey E, Muñoz PG, Fernández-Palacios JM, 2010. The effect of fire severity on first-year seedling establishment in a Pinus canariensis forest on Tenerife, Canary Islands. Eur J For Res 129: 499-508. https://doi.org/10.1007/s10342-009-0347-6
  • Pereira C, Montalbán IA, García-Mendiguren O, Goicoa T, Ugarte MD, Correia S, Canhoto JM, Moncaleán P, 2016. Pinus halepensis somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process. J For Res 21: 143-150. https://doi.org/10.1007/s10310-016-0524-7
  • Pereira C, Montalbán IA, Goicoa T, Ugarte MD, Correia S, Canhoto JM, Moncaleán P, 2017. The effect of changing temperature and agar concentration at proliferation stage in the final success of aleppo pine somatic embryogenesis. For Syst 26: 1-4. https://doi.org/10.5424/fs/2017263-11436
  • Quoirin M, Lepoivre P, 1977. Études des milieux adaptés aux cultures in vitro de Prunus. Acta Hort 78: 437-442. https://doi.org/10.17660/ActaHortic.1977.78.54
  • Walter C, Find JI, Grace LJ, 2005. Somatic embryogenesis and genetic transformation, in Pinus radiata. In: Jain SM, Gupta PK (eds) Protocols for somatic embryogenesis in woody plants. Forestry Sciences, vol 77. Springer, Dordrecht, pp 491-504. https://doi.org/10.1007/1-4020-2985-3_2
Fuente de los datos: Dialnet