Pinus canariensis plant regeneration through somatic embryogenesis

  1. Ander Castander-Olarieta 1
  2. Paloma Moncaleán 1
  3. Itziar A. Montalbán 1
  1. 1 Department of Forestry Science, NEIKER-Tecnalia, Arkaute
Aldizkaria:
Forest systems

ISSN: 2171-5068

Argitalpen urtea: 2020

Alea: 29

Zenbakia: 1

Orrialdeak: 61-66

Mota: Artikulua

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Beste argitalpen batzuk: Forest systems

Laburpena

Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis. Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain). Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions. Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth. Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.

€ Ikusi finantzaketa

Finantzaketari buruzko informazioa

MINECO (Spanish Government) AGL2016-76143-C4-3R CYTED P117RT0522 DECO (Basque government) Ayudas de formaci?n a j?venes investigadores y tecn?logos Special mention to Enrique Surroca, who actively helped with the collection of plant material.

Finantzatzaile

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