The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesisShort communication

  1. Pereira, Catia
  2. Montalbán, Itziar A.
  3. Goicoa, Tomás
  4. Ugarte, Dolores
  5. Correia, Sandra
  6. Canhoto, Jorge M.
  7. Moncaleán, Paloma
Revista:
Forest systems

ISSN: 2171-5068

Año de publicación: 2017

Volumen: 26

Número: 3

Tipo: Artículo

DOI: 10.5424/FS/2017263-11436 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Forest systems

Objetivos de desarrollo sostenible

Resumen

Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis.Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain).Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage.Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages.Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. 

Referencias bibliográficas

  • Teyssier C, Grondin C, Bonhomme L, Lomenech AM, Vallance M, Morabito D, Label P, Lelu-Walter MA, 2011. Increased gelling agent concentration promotes somatic embryo maturation in hybrid larch (Larix x eurolepsis): a 2-DE proteomic analysis. Physiol Plantarum 141:152-165. https://doi.org/10.1111/j.1399-3054.2010.01423.x
  • Jeddi K, Cortina J, Chaieb M, 2009. Acacia salicina, Pinus halepensis and Eucalyptus occidentalis improve soil surface conditions in arid southern Tunisia. J Arid Environ 73: 1005-1013. https://doi.org/10.1016/j.jaridenv.2009.05.005
  • Kvaalen H, Johnsen Ø, 2008. Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytol 177: 49-59.
  • Maestre FT, Cortina J. 2004. Are Pinus halepensis plantations useful as a restoration tool in semiarid Mediterranean areas? Forest Ecol Manag 198: 303-317. https://doi.org/10.1016/j.foreco.2004.05.040
  • Montalbán IA, De Diego N, Moncaleán P, 2012. Enhancing initiation and proliferation in radiata pine (Pinus radiata D. Don) somatic embryogenesis through seed family screening, zygotic embryo staging and media adjustments. Acta Physiol Plant 34: 451-460. https://doi.org/10.1007/s11738-011-0841-6
  • Montalbán IA, Setién-Olarra A, Hargreaves CL, Moncaleán P, 2013. Somatic embryogenesis in Pinus halepensis Mill.: an important ecological species from the Mediterranean forest. Trees-Struct Funct 27: 1339-1351. https://doi.org/10.1007/s00468-013-0882-0
  • Montalbán IA, García-Mendiguren O, Goicoa T, Ugarte MD, Moncaleán P, 2015. Cold storage of initial plant material affects positively somatic embryogenesis in Pinus radiata. New Forest 46: 157-165. https://doi.org/10.1007/s11056-014-9457-1
  • Morel A, Teyssier C, Trontin JF, Eliásová K, Pesĕk B, Beaufour M, Morabito D, Boizot N, Le Metté C, Belal-Bessai L et al., 2014. Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses. Physiol Plantarum 152: 184-201. https://doi.org/10.1111/ppl.12158
  • Pereira C, Montalbán IA, García-Mendiguren O, Goicoa T, Ugarte D, Correia S, Canhoto JM, Moncaleán P. 2016. Pinus halepensis somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process. J For Res-Jpn 21: 143-150. https://doi.org/10.1007/s10310-016-0524-7
  • Pullman GS, Bucalo K, 2014. Pine somatic embryogenesis: analyses of seed tissue and medium to improve protocol development. New Forest 45: 353-377. https://doi.org/10.1007/s11056-014-9407-y
  • Teyssier C, Grondin C, Bonhomme L, Lomenech AM, Vallance M, Morabito D, Label P, Lelu-Walter MA, 2011. Increased gelling agent concentration promotes somatic embryo maturation in hybrid larch (Larix x eurolepsis): a 2-DE proteomic analysis. Physiol Plantarum 141:152-165. https://doi.org/10.1111/j.1399-3054.2010.01423.x
Fuente de los datos: Dialnet