The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesisShort communication

  1. Pereira, Catia
  2. Montalbán, Itziar A.
  3. Goicoa, Tomás
  4. Ugarte, Dolores
  5. Correia, Sandra
  6. Canhoto, Jorge M.
  7. Moncaleán, Paloma
Journal:
Forest systems

ISSN: 2171-5068

Year of publication: 2017

Volume: 26

Issue: 3

Type: Article

DOI: 10.5424/FS/2017263-11436 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Forest systems

Sustainable development goals

Abstract

Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis.Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain).Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage.Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages.Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. 

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