Numerical Methods of Cryo Electron Tomography Laboratory
Responsable: Daniel Castaño Díez
Centre: IBF Instituto Biofisika
Page web: https://www.biofisika.org/en/research/numerical-methods-cryo-electron-tomography-laboratory
We develop software and algorithmic methods for cryo-electron tomography (cryoET). Our inhouse package Dynamo covers all stages of this computational pipeline: alignment of tilt series, reconstruction of tomograms, particle extraction and subtomogram averaging and classification. Using constitutive exocytosis in yeast cells as experimental example, we ultimately aim at building a numerical infrastructure for the combined use of cryoET, intracellular nanotools and live cell imaging to resolve dynamic cellular processes with atomic resolution.
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- Centres: IBF Instituto Biofisika