LARGE EQUIPMENT: Stoelting stereotaxic surgery set up with Nanoject microcapillary injector; Leica vibratome; Heracell tissue culture incubator; Perimed Doppler cerebral blood flow analyzer.
TECHNIQUES: Stereotaxic surgery, immunofluorescence, epifluorescent and confocal microscopy and electron microscopy, stereology-based quantification, qPCR, gene arrays, ChiPSeq, RNASeq, fluorescent-activated cell sorting, Western blot, Seahorse metabolic analysis, primary cultures, organotypic cultures, in vitro models of phagocytosis, iPSCs.
