TECHNIQUES: Cell culture, including immortalized cell lines, human embryonic neural stem cells, induced pluripotent stem cells and primary neural cells. Microfluidics of primary neural cultures and induced pluripotent stem cells. Human organoid culture of induced pluripotent stem cells. Tissue processing for in situ hybridization, immunofluorescence, immunohistochemistry and proximity ligation assays. Generation and quality control of protein aggregates. Fluorescent and confocal microscopy. RNAseq sampling and analysis.
