Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson's GBA-N370S mutation

  1. Do, Quyen B. 1
  2. Ng, Brian 2
  3. Marquez Gomez, Ricardo 1
  4. Beccano-Kelly, Dayne 2
  5. Ibarra-Aizpura, Naroa 2
  6. Caiazza, Maria-Claudia 1
  7. Lang, Chairmaine 1
  8. Baleriola, Jimena 3
  9. Bengoa-Vergniory, Nora 4
  10. Wade-Martins, Richard 1
  1. 1 Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, United Kingdom; Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, United Kingdom; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA
  2. 2 Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, United Kingdom; Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, United Kingdom
  3. 3 Achucarro Basque Center for Neuroscience, Leioa, Spain; Ikerbasque - Basque Foundation for Science, Bilbao, Spain
  4. 4 Oxford Parkinson's Disease Centre and Department of Physiology, Anatomy and Genetics, University of Oxford, South Park Road, Oxford OX1 3QU, United Kingdom; Kavli Institute for Neuroscience Discovery, University of Oxford, Dorothy Crowfoot Hodgkin Building, South Park Road, Oxford OX1 3QU, United Kingdom; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA; Achucarro Basque Center for Neuroscience, Leioa, Spain; Ikerbasque - Basque Foundation for Science, Bilbao, Spain; Ikerbasque - Basque Foundation for Science, Bilbao, Spain
Mostrar afiliaciones +

Editor: Zenodo

Año de publicación: 2023

Tipo: Dataset

CC BY 4.0

DOI: 10.5281/ZENODO.7661355 lock_openAcceso abierto editor

Resumen

<strong>ABSTRACT</strong> Understanding medium spiny neuron (MSN) physiology is essential to understand motor impairments in Parkinson’s disease (PD) given the architecture of the basal ganglia. Here, we developed a custom three-chamber microfluidic platform and established a cortico-striato-nigral microcircuit recapitulating the striatal presynaptic triad <em>in vitro</em> using induced pluripotent stem cell (iPSC)-derived neurons. We found that, although cortical glutamatergic projections facilitated MSN synaptic activity, dopaminergic transmission was essential for excitability maturation of MSNs <em>in vitro</em>. Replacement of wild-type iPSC-dopamine neurons (iPSC-DaNs) in the striatal microcircuit with those carrying the PD-related <em>GBA-N370S</em> mutation induced early hyperexcitability in iPSC-MSNs through reduction of voltage-gated sodium and potassium intrinsic currents. Such deficits were resolved in aged cultures or with antagonism of protein kinase A activity in nigrostriatal iPSC-DaNs. Hence, our results highlight the unique utility of modelling striatal neurons in a modular and highly physiological circuit which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of <em>GBA1</em> PD patients. <strong>FILE DESCRIPTIONS</strong> Source Data.xlsx: Tabular datasets plotted on main figures 1, 3, 4, 5 and 6. Supplementary Data.xlsx: Tabular datasets plotted on supplementary figures 1, 2 and 3. Key Resources Table.xlsx: Table containing key resources (primary and secondary antibodies, cell lines and software) used in this study. List of Primers.xlsx: Primers used in RT-qPCR.