Targeted Photodynamic Therapy: Gluconamide-Modified Cellulose Nanocrystals as Efficient Photosensitizer Delivery Platforms Against Gram-Negative Bacteria

  1. Prieto-Montero, Ruth 24
  2. Tejón, Maite 24
  3. Albaya, Andrea 1
  4. Arbeloa, Teresa 24
  5. Chiara, Jose Luis 1
  6. L. Fanárraga, Mónica 3
  7. MARTINEZ-MARTINEZ, VIRGINIA 24
  1. 1 Instituto de Química Orgánica General (IQOG-CSIC)
  2. 2 Universidad del País Vasco, UPV/EHU
  3. 3 Nanomedicina-IDIVAL, Universidad de Cantabria
  4. 4 Universidad del País Vasco/Euskal Herriko Unibertsitatea
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    Universidad del País Vasco/Euskal Herriko Unibertsitatea

    Lejona, España

    ROR https://ror.org/000xsnr85

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Revista:
ChemRxiv

Año de publicación: 2023

Tipo: Documento de Trabajo

DOI: 10.26434/CHEMRXIV-2023-2JX24 GOOGLE SCHOLAR lock_openAcceso abierto editor

Resumen

Antimicrobial Photodynamic Therapy (aPDT) is an emerging strategy to fight against resistant pathogenic bacteria, which is considered a serious health problem. In this work, we described the functionalization of cellulose nanocrystals (CNC) with two photosensitizers (PS): one cationic and the other anionic, Rose Bengal (RB) and Toluidine Blue O (TBO), respectively, together with a specific target, gluconamide, as potential nanosystem for a PDT against Gram-negative bacteria such as Escherichia Coli (E. coli). The nanosystems loaded with the photosensitizers (PS@CNC) are chemically and photophysically characterized and tested in E. coli bacteria comparing their cyto- and phototoxicity with the PSs in solution. The resultant PS@CNC have reduced the inherent cytotoxicity of PSs in dark conditions maintaining their phototoxicity activity under green and red irradiation for RB@CNC and TBO@CNC, respectively. The presence of gluconamide as the target has improved the phototoxic of the nanosystem.