Development of gene-activated matrices as tools in new regenerative therapies

  1. Rilo Alvarez, Héctor
Zuzendaria:
  1. Marcos García Fuentes Zuzendaria
  2. Anxo Vidal Figueroa Zuzendaria

Defentsa unibertsitatea: Universidade de Santiago de Compostela

Defentsa urtea: 2024

Epaimahaia:
  1. Rosa María Hernández Martín Presidentea
  2. Luis Antonio Díaz Gómez Idazkaria
  3. María del Carmen Arufe Gonda Kidea

Mota: Tesia

Teseo: 828438 DIALNET lock_openMINERVA editor

Laburpena

The main goal of this thesis has been the development, optimization, and testing of three-dimensional mRNA-activated matrices as a system for the administration of transcription factors for articular cartilage regeneration. Modifications in the mRNA synthesis provided higher stability and efficacy as compared to standard mRNA. TAMs chondrogenic effects were tested in 3D cultures activated with SOX9, TGF-β3 or combinations of both. The transcript combination provided the best effects as it combined the strong chondrogenic effect of TGF-β3 with the capacity of SOX9 to promote chondroblast differentiation while inhibiting fibrotic tissue formation. The two best TAM prototypes were studied in a cartilage defect rabbit model where showed no signs of toxicity and promoted the expression of specific tissue markers.