MARK4ren interakzioak USP21 deubikuitinasaren kokapen azpizelular dinamikoa erregulatzen du

  1. García-Santisteban, Iraia 1
  2. Olazabal-Herrero, Anne 2
  3. Rodríguez, Jose Antonio 3
  1. 1 Genetika, Antropologia Fisikoa eta Animalien Fisiologia Saila (UPV/EHU), Leioa Biocruces-Bizkaia Osasun Ikerketa Institutua, Barakaldo
  2. 2 Department of Oncology, Georgetown University, Washington, DC Department of Internal Medicine, Yale University School of Medicine, New Haven, CT
  3. 3 Genetika, Antropologia Fisikoa eta Animalien Fisiologia Saila (UPV/EHU), Leioa
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Journal:
Ekaia: Euskal Herriko Unibertsitateko zientzi eta teknologi aldizkaria

ISSN: 0214-9001

Year of publication: 2022

Issue: 42

Pages: 193-206

Type: Article

DOI: 10.1387/EKAIA.22644 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Ekaia: Euskal Herriko Unibertsitateko zientzi eta teknologi aldizkaria

Abstract

The deubiquitinase USP21 shuttles between the nucleus and the cytoplasm and can also localize to the centrosome, but how this complex subcellular localization is modulated remains unknown. A proteomics analysis has identified the centrosomal kinase MARK4 as a potential USP21-interacting partner. In this work, we validate the USP21/MARK4 interaction, identifying the MARK4-binding region of USP21. Our results indicate that MARK4 interaction may regulate USP21 nucleocytoplasmic shuttling dynamics, increasing its retention in the cytoplasm. On the other hand, our data show that the centrosomal localization of both proteins is highly dynamic, and suggest that USP21 centrosomal dynamics might be modulated by MARK4.

Data source: Dialnet