Identification of candidate genes involved in the response to different abiotic stresses in potato (Solanum tuberosumL.)
- L. Barandalla
- A. Álvarez
- J.I. Ruiz de Galarreta
- E.Ritter
ISSN: 1019-6609, 1853-4961
Año de publicación: 2018
Volumen: 22
Número: 2
Páginas: 33-38
Tipo: Artículo
Otras publicaciones en: Revista Latinoamericana de la Papa
Resumen
Plants growing in natural habitats are exposed to multiple environmental stresses resulting from abiotic factors such as heat, drought, and cold, which have a significant impact on cultivated potato. We have evaluated in two Solanum tuberosumvarieties, Soprano and Kondor, the adaptation to different abiotic stresses as heat, cold, and drought. For this purpose plants of both varieties were stressed, and when they showed symptoms, RNA extraction was carried out and a cDNA library for each sample was constructed. The objective of this study was to detect and analyse the genes involved in the responses to abiotic stresses in potato.The assay generated transcriptome sequences from both varieties, and a total of 5.579.655 reads and 8420 putative candidate genes were generated. 4.027 of the candidate genes were polymorphic and presented a different number of patterns defined by a varying number of SNPs. Many of the generated candidate genes showed differential expression, since the candidate gene was present in the stressed plant, but not in the control plant. The application of this methodology allows us to detect numerous candidate genes or specific alleles/allele combinations, which are differentially expressed in specific samples after the application of different abiotic stresses. This will be useful to identify superior alleles which can be used in Marker Assisted Selection for resistance and tolerance to abiotic stresses
Referencias bibliográficas
- Bachem CWB,Oomen RJFJ, Visser RGF. 1998. Transcript imaging with cDNA-AFLP: a step-by-step protocol. Plant Mol Biol Rep. 16:157-173.
- Monneveux P, Ramírez DA, Pino M-T. 2013. Drought tolerance in potato (S. tuberosumL.): can we learn from drought tolerance research in cereals? Plant Sci 205–206:76–86.
- Qunfeng W, Liurong F, Xuebao W, Bin L, Rui L, Zhengjun Y. 2009. A pseudotype baculovirus-mediated vaccine confers protective immunity against lethal challenge with H5N1 avian influenza virus in mice and chickens. Molecular Inmunology 46: 2210-7.
- Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Nova York.
- Simelton E, Fraser EDG, Termansen M, Benton TG, Gosling SN, South A, Arnell NW, Challinor AJ, Dougill AJ, Forster PM. 2012.The socioeconomics of food crop production and climate change vulnerability: a global scale quantitative analysis of how grain crops are sensitive to drought. Food Security 4:163–179.
- Slater JW. 1968. The effect of night temperature on tuber initiation of the potato. Eur Potato J 11:14–22.
- Wang-Pruski G, Schofield A. 2012. Potato: Improving crop productivity and abiotic stress tolerance. In: Tuteja N, Gill SS, Tiburcio AF, Tuteja R, editors. Improving crop resistance to abiotic stress. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. p.1121–1153.
- Yuan BZ, Nishiyama S, Kang Y. 2003. Effects of different irrigation regimes on the growth and yield of drip-irrigated potato. Agric. Water Manage. 63:153–167.