Winter biodisinfestation with Brassica green manure is a promising management strategy for Phytophthora capsici control of protected pepper crops in humid temperate climate regions of northern Spain

  1. Maite Gandariasbeitia 1
  2. Mikel Ojinaga 1
  3. Estibaliz Orbegozo 1
  4. Amaia Ortíz-Barredo 1
  5. Mireia Núñez-Zofío 1
  6. Sorkunde Mendarte 2
  7. Santiago Larregla 1
  1. 1 NEIKER-Basque Institute for Agricultural Research and Development, Plant Production and Protection Dept. Derio (Bizkaia), Spain
  2. 2 NEIKER, Conservation of Natural Resources Dept. Derio (Bizkaia), Spain
Revista:
Spanish journal of agricultural research

ISSN: 1695-971X 2171-9292

Año de publicación: 2019

Volumen: 17

Número: 1

Tipo: Artículo

DOI: 10.5424/SJAR/2019171-13808 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Spanish journal of agricultural research

Objetivos de desarrollo sostenible

Resumen

Phytophthora capsici causes root and crown rot of protected pepper (Capsicum annuum L.) crops in the Basque Country (northern Spain), a humid temperate climate area. The objective was to determine the effect of winter biofumigation and plastic cover (biodisinfestation) with Sinapis alba L. (cultivar 'Ludique') fresh green manure on the survival of introduced P. capsici inoculum (oospores) in a greenhouse soil. After autumn-winter Brassica cover crop soil incorporation in February, oospores remained at 15 and 30 cm depth for four weeks´ time exposure in two consecutive years, 2009 and 2010. Oospores viability was estimated with a plasmolysis method and infectivity with a potted pepper bioassay. Viability was significantly higher in biodisinfestated oospores than in the non-treated control in both years (81% and 21% relative increase in 2009 and 2010, respectively) and significantly higher at 30 cm depth than at 15 cm in 2009 (24% relative increase). Conversely, biodisinfestation significantly delayed disease incidence progression until the first half of infectivity bioassays in both years compared to the non-treated soils (83% and 75% relative decrease of dead plants in 2009 and 2010 respectively). The low soil temperatures could explain the low oospore survival reduction and suggest that the suppressiveness expressed in the infectivity bioassay was related with an increase of microbial activity in the biodisinfestation treatment. We conclude that winter biodisinfestation with fresh Brassica green manure is a promising management strategy for Phytophthora root rot control of protected peppers crops in regions of humid temperate climate such us northern Spain.

Información de financiación

FEDER funds (projects INIA RTA 2011-00005-C03-03 and INIA RTA 2015-00060-C04-04); Basque Government, Dept. of Environment, Territorial Planning, Agriculture and Fisheries (projects BIOSOL and REVABIO). MG was the recipient of a predoctoral contract (INIA-2017-0043) of the State Training Subprogram of the Spanish Ministry of Economy, Industry and Competitiveness (MICINN); EO was the recipient of a technologist scholarship "Disease management in protected horticultural organic crops" (Order of October 18, 2017 of the Minister of Economic Development and Competitiveness of the Basque Government). The authors thank Berdaitz Juaristi, Javier Elorrieta and Leire Abaunza for their helpful support to carry out greenhouse experimental field trials in NEIKER.

Referencias bibliográficas

  • Angus JF, Gardner PA, Kirkegaard JA, Desmancheiler JM, 1994. Biofumigation: Isothiocyanates released from Brassica roots inhibit the growth of take-all fungus. Plant Soil 162: 107-112. https://doi.org/10.1007/BF01416095
  • Arriaga H, Núñez-Zofío M, Larregla S, Merino P, 2011. Gaseous emissions from soil biodisinfestation by animal manure on a greenhouse pepper crop. Crop Prot 30: 412-419. https://doi.org/10.1016/j.cropro.2010.12.012
  • Bending G, Lincoln SD, 1999. Characterisation of volatile sulphur containing compounds produced during decomposition of Brassica juncea tissues in soil. Soil Biol Biochem 31: 695-703. https://doi.org/10.1016/S0038-0717(98)00163-1
  • Bowers JH, Mitchell DJ, 1991. Relationship between inoculum level of Phytophthora capsici and mortality of pepper. Phytopathology 81: 178-184. https://doi.org/10.1094/Phyto-81-178
  • Chandrashekara C, Bhatt JC, Kumar R, Chandrashekara KN, 2014. Supressive soils in plant disease management. In: Eco-friendly innovative approaches in plant disease management; Singh VK, Singh Y, Singh A (eds.). pp: 241-256. Int Book Distrib & Publ, New Delhi.
  • Coelho L, Chellemi DO, Mitchell DJ, 1999. Efficacy of solarization and cabbage amendment for the control of Phytophthora spp. in North Florida. Plant Dis 83: 293-299. https://doi.org/10.1094/PDIS.1999.83.3.293
  • Cohen MF, Yamasaki H, Mazzola M, 2005. Brassica napus seed meal soil amendment modifies microbial community structure, nitric oxide production and incidence of Rhizoctonia root rot. Soil Biol Biochem 37: 1215-1227. https://doi.org/10.1016/j.soilbio.2004.11.027
  • Colla P, Gilardi G, Gullino ML, 2012. A review and critical analysis of the European situation of soil-borne disease management in the vegetable sector. Phytoparasitica 40: 515-523. https://doi.org/10.1007/s12600-012-0252-2
  • Cook RJ, Baker KF, 1983. The nature and practice of biological control of plant pathogens. APS Press, St. Paul, MN, USA. 539 pp.
  • De-Cara-García M, Fernández-Plaza M, Gómez-Vázquez J, 2018. Pathogenic and biological characterization of Phytophthora capsici isolates from zucchini and pepper in Southeast Spain. Span J Agric Res 16 (2): e1005. https://doi.org/10.5424/sjar/2018162-13129
  • Erwin DC, Ribeiro OK (eds.), 1996. Phytophthora diseases worldwide. Am Phytopathol Soc, APS Press, St. Paul, MN, USA. 562 pp.
  • Etxeberria A, Mendarte S, Larregla S, 2011a. Determination of viability of Phytophthora capsici oospores with the tetrazolium bromide staining test versus a plasmolysis method. Rev Iberoam Micol 28 (1): 43-49. https://doi.org/10.1016/j.riam.2010.11.005
  • Etxeberria A, Mendarte S, Larregla S, 2011b. Thermal inactivation of Phytophthora capsici oospores. Rev Iberoam Micol 28 (2): 83-90. https://doi.org/10.1016/j.riam.2011.01.004
  • Gamliel A, Stapleton JJ, 1993. Characterization of antifungal volatile compounds evolved from solarized soil amended with cabbage residues. Phytopathology 83: 899-905. https://doi.org/10.1094/Phyto-83-899
  • Guerrero MM, Ros C, Lacasa CM, Martínez V, Lacasa A, Fernández P, Martínez MA, Núñez-Zofío M, Larregla S, Díez-Rojo MA, Bello A, 2010. Effect of biosolarization using pellets of Brassica carinata on soil-borne pathogens in protected pepper crops. Acta Hortic 883: 337-344. https://doi.org/10.17660/ActaHortic.2010.883.42
  • Guerrero MM, Lacasa CM, Hernández A, Martínez V, Martínez MC, Fernández-Molina P, Lacasa A, 2014. Biosolarization with agroindustrial byproduct for the control of soil-borne pathogens in protected pepper crops in Southeast Spain. Acta Hortic 1044: 157-161. https://doi.org/10.17660/ActaHortic.2014.1044.19
  • Hewavitharana SS, Mazzola M, 2016. Carbon source-dependent effects of anaerobic soil disinfestation on soil microbiome and suppression of Rhizoctonia solani AG-5 and Pratylenchus penetrans. Phytopathology 106: 1015-1028. https://doi.org/10.1094/PHYTO-12-15-0329-R
  • Janvier C, Villeneuve F, Alabouvette C, Edel-Hermann V, Mateille T, Steinberg C, 2007. Soil health through soil disease suppression: which strategy from descriptors to indicators? Soil Biol Biochem 39: 1-23. https://doi.org/10.1016/j.soilbio.2006.07.001
  • Jeffers SN, Martin SB, 1986. Comparison of two media selective for Phytophthora and Pythium species. Plant Dis 70: 1038-1043. https://doi.org/10.1094/PD-70-1038
  • Jiang J, Erwin DC, 1990. Morphology, plasmolysis, and tetrazolium bromide stain as criteria for determining viability of Phytophthora oospores. Mycologia 82: 107-113. https://doi.org/10.1080/00275514.1990.12025847
  • Katan J, Gamliel A, 2014. Plant health management: Soil solarization. In: Encyclopedia of Agriculture and Food Systems, pp: 460-471.
  • Kirkegaard JA, Sarwar M, 1998. Biofumigation potential of brassicas I. Variation in glucosinolate profiles of diverse field-grown brassicas. Plant Soil 201: 71-89. https://doi.org/10.1023/A:1004364713152
  • Kirkegaard JA, Angus JF, Gardner PA, Cresswell HP, 1993. Benefits of brassica break crops in the Southeast wheatbelt. Proc. 7th Aust Agron Conf, Adelaide, South Australia, Sept 19-24. pp 19-24.
  • Kirkegaard JA, Matthiessen JN, 2004. Developing and refining the biofumigation concept. Agroindustria 3: 233-239.
  • Lacasa CM, Guerrero MM, Ros C, Martínez V, Lacasa A, Fernández P, Núñez-Zofío M, Larregla S, Díez-Rojo MA, Bello A, 2010. Efficacy of biosolarization with sugar beet vinasses for soil disinfestation in pepper greenhouses. Acta Hortic 883: 345-352. https://doi.org/10.17660/ActaHortic.2010.883.43
  • Lacasa CM, Martínez V, Hernández A, Ros C, Lacasa A, Guerrero MM, Rodriguez-Molina MC, Serrano-Pérez P, Larregla S, 2015. Survival reduction of Phytophthora capsici oospores and P. nicotianae chlamydospores with Brassica green manures combined with solarization. Sci Hortic 197: 607-618. https://doi.org/10.1016/j.scienta.2015.10.024
  • Larkin RP, Griffin TS, 2007. Control of soilborne potato diseases using Brassica green manures. Crop Prot 26 (7): 1067-1077. https://doi.org/10.1016/j.cropro.2006.10.004
  • Larkin RP, 2013. Green manures and plant disease management. Review. CAB Reviews 8, No. 037.
  • Larregla S, Guerrero MM, Mendarte S, Lacasa A, 2015. Biodisinfestation with organic amendments for soil fatigue and soil-borne pathogens control in protected pepper crops. In: Organic amendments and soil suppressiveness in plant disease management; Meghvansi MK, Varma A (eds.). pp: 437-456. Springer Int Publ, Switzerland. https://doi.org/10.1007/978-3-319-23075-7_21
  • Madden LV, Hughes G, Van Den Bosch F, 2007. Temporal analysis I: Quantifying and comparing epidemics. In: The study of plant disease epidemics; Madden LV, Hughes G, Van Den Bosch F (eds.). pp: 63-116. APS Press, St. Paul, MN, USA.
  • Manici LM, Lazzeri L, Baruzzi G, Leoni O, Galletti S, Palmieri S, 2000. Suppressive activity of some glucosinolate enzyme degradation products on Pythium irregulare and Rhizoctonia solani in sterile soil. Pest Manag Sci 56: 921-926. https://doi.org/10.1002/1526-4998(200010)56:10<921::AID-PS232>3.0.CO;2-L
  • Matthiessen JN, Kirkegaard JA, 2006. Biofumigation and enhanced biodegradation: opportunity and challenge in soil-borne pest and disease management. CRC Crit Rev Plant Sci 25: 235-265. https://doi.org/10.1080/07352680600611543
  • Mazzola M, Granatstein DM, Elfving DC, Mullinix K, 2001. Suppression of specific apple root pathogens by Brassica napus seed meal amendment regardless of glucosinolate content. Phytopathology 91: 673-679. https://doi.org/10.1094/PHYTO.2001.91.7.673
  • Mazzola M, Agostini A, Cohen MF 2017. Incorporation of Brassica seed meal soil amendment and wheat cultivation for control of Macrophomina phaseolina in strawberry. Eur J Plant Pathol 149: 57-71. https://doi.org/10.1007/s10658-017-1166-0
  • Mazzola M, Muramoto J, Shennan C. 2018. Anaerobic disinfestation induced changes to the soil microbiome, disease incidence and strawberry fruit yields in California field trials. Appl Soil Ecol 127: 74-86. https://doi.org/10.1016/j.apsoil.2018.03.009
  • Mcguire AN, 2003. Mustard green manures replace fumigant and improve infiltration in potato cropping system. Crop Manag 2 (1): 105-112. https://doi.org/10.1094/CM-2003-0822-01-RS
  • Morales-Rodríguez C, Palo C, Palo E, Rodríguez-Molina MC, 2014. Control of Phytophthora nicotianae with Mefenoxam, fresh Brassica tissues, and Brassica pellets. Plant Dis 98: 77-83. https://doi.org/10.1094/PDIS-04-13-0393-RE
  • Núñez-Zofío M, Garbisu C, Larregla S, 2011a. Application of organic amendments followed by plastic mulching for the control of Phytophthora root rot of pepper in northern Spain. Acta Hortic 883: 353-360.
  • Núñez-Zofío M, Garbisu C, Larregla S, 2011b. Application of organic amendments followed by soil plastic mulching reduces the incidence of Phytophthora capsici in pepper crops under temperate climate. Crop Prot 30: 1563-1572. https://doi.org/10.1016/j.cropro.2011.08.020
  • Núñez-Zofío M, Larregla S, Garbisu C, 2012. Repeated biodisinfestation controls the incidence of Phytophthora root and crown rot of pepper while improving soil quality. Span J Agric Res 10: 794-805. https://doi.org/10.5424/sjar/2012103-571-11
  • OJ, 2009. Directive 2009/128/EC of the European Parliament and of the Council of 21 October 2009 establishing a framework for Community action to achieve the sustainable use of pesticides. Official Journal L 309, 24.11.2009, pp: 71-86.
  • Pittis JE, Shattock RC, 1994. Viability, germination and infection potential of oospores of Phytophthora infestans. Plant Pathol 43: 387-396. https://doi.org/10.1111/j.1365-3059.1994.tb02700.x
  • Prasad P, Kumar J, Pandey S, 2015. Biofumigation: Success and prospects in soilborne plant disease management. JAPSA 1 (6): 47-59.
  • Prasad P, Kumar J, Pandey S, 2016. Investigating disease controlling ability of brassica volatiles and their compatibility with Trichoderma harzianum. Proc Nat Acad Sci, India B: Biol Sci 88 (3).
  • Riga P, Goikoetxea X, Larregla S, 2000. In vitro toxicity of HNO2 and NH3 in Phytophthora capsici mycelium growth. Acta Hortic 532: 225-228. https://doi.org/10.17660/ActaHortic.2000.532.30
  • Ristaino JB, Johnston SA, 1999. Ecologically based approaches to management of Phytophthora blight on bell pepper. Plant Dis 83 (12): 1080-1087. https://doi.org/10.1094/PDIS.1999.83.12.1080
  • Rodríguez-Molina MC, Serrano‐Pérez P, Palo C, 2016. Effect of biofumigation with brassica pellets combined with Brassicaceae cover crops and plastic cover on the survival and infectivity of inoculum of Phytophthora nicotianae Breda de Haan. Pest Manag Sci 72: 1295-1301. https://doi.org/10.1002/ps.4144
  • Rudolph RE, Sams C, Steiner R, Thomas SH, Walker S, Uchanski ME, 2015. Biofumigation performance of four brassica crops in a green chile pepper (Capsicum annuum) rotation system in Southern New Mexico. Hortscience 50: 247-253. https://doi.org/10.21273/HORTSCI.50.2.247
  • Sarwar M, Kirkegaard JA, 1998. Biofumigation potential of brassicas. Plant Soil 201: 91-101. https://doi.org/10.1023/A:1004333230899
  • Shurtleff MC, Averre CV, 1997. Glossary of plant-pathological terms. APS Press, St. Paul, MN, USA, 361 pp.
  • Wang D, Rosen C, Kinkel L, Cao A, Tharayil N, Gerik J, 2009. Production of methyl sulfide and dimethyl disulfide from soil-incorporated plant materials and implications for controlling soil-borne pathogens. Plant Soil 324: 185-197. https://doi.org/10.1007/s11104-009-9943-y