Synthesis of n-glycan mimetics and their evaluation as c-type lectin receptor (clr) antagonists using microarray technology

  1. Cioce, Anna
Dirigida por:
  1. Niels-Christian Reichardt Director
  2. Claudio Palomo Nicolau Director/a
  3. Jesús Jiménez Barbero Director/a

Universidad de defensa: Universidad del País Vasco - Euskal Herriko Unibertsitatea

Fecha de defensa: 01 de febrero de 2019

Tribunal:
  1. Mikel Oiarbide Presidente
  2. Francisco Javier Rojo Marcos Secretario/a
  3. Sabine L. Flitsch Vocal

Tipo: Tesis

Teseo: 148748 DIALNET lock_openADDI editor

Resumen

Carbohydrate microarrays are well established tools for high-throughput analysis of carbohydrate-protein interactions and for screening the specificity of carbohydrate processing enzymes, In addition, a few groups have also explored the on-chip synthesis of ligands. The use of small amounts of substrates and the absence of long purification step make the microarray a time-saving approach for the parallel synthesis of hundred scaffolds.We developed a versatile platform to quickly synthesize significant number of glycomimetics via chemoenzymatic protocols and test them as potential ligands for C-type lectin receptors (CLRs). The transparent and the conductive hydrophobic-ITO surface allows to perform binding studies with labeled lectins by fluorescent spectroscopy as well as to estimate conversion rates of the on-chip reactions for every printed ligand by MALDI-TOF MS.