Structural study of CUG-repeating small RNAs complexed with silencing suppressor P19

  1. Katorcha ---, Elizaveta
Dirigida por:
  1. Lioudmila Malinina Director/a

Universidad de defensa: Universidad del País Vasco - Euskal Herriko Unibertsitatea

Fecha de defensa: 07 de marzo de 2012

Tribunal:
  1. Félix María Goñi Urcelay Presidente
  2. Marcelo Eduardo Guerin Secretario
  3. Miquel Coll Capella Vocal
  4. Josefa de Lourdes Campos Lopez Vocal
  5. Juan Antonio Subirana Torrent Vocal

Tipo: Tesis

Teseo: 115719 DIALNET lock_openADDI editor

Resumen

The study is focused on structural aspects of interaction between silencing suppressor p19 and CUG-repeating small RNAs. The work involves crystal structure determination of a protein-unbound RNA form and RNA fragments of various lengths (19, 20, 21 nucleotides) complexed with p19-suppressor. Results prove the ability of silencing suppressor p19 to bind CUG-repeating small RNAs, as well as reveal features of U•U mismatches flanked by Watson-Crick C•G base pairs in p19-bound and p19-unbound states. In addition, structural data reveal a p19 specific site for anchoring extra nucleotides in small RNAs. In general, the study extends our knowledge about the mechanism of small RNA recognition by silencing suppressor p19.