Proteinetan oinarritutako biomaterialen biobateragarritasunaren eta egokitasunaren azterketa kornearakoordezkoen edo matrizeen hautagai potentzial gisa

Resumo

The current study has focused on the in vitro assessment of protein-based biomaterials that could be a potentialsource for corneal scaffolds. Collagen, soy protein isolate (SPI) and gelatin films cross-linked with lactose or citricacid were prepared and transmittance and degradation measurements were carried out. In vitro cytotoxicity, celladhesion and migration studies were performed with human corneal epithelial cells (HCE) and 3T3 fibroblaststo assess the biocompatibility of the films. Transmittance values met the needs of the cornea and the degradationprofile revealed a progressive decomposition of the biomaterials. Cell viability at 72 h was above 70% whenexposed to SPI and gelatin films. Adhesion analysis demonstrated the adhesion of both cell types to the films, witha similar arrangement to that observed in controls. Besides, both cell lines were able to proliferate and migrateover the films. These results demonstrated that the studied biomaterials could be potential alternatives applicablein corneal bioengineering.